Derivation of canine hepatocyte in vitro models to study Branched-Chain Amino Acid effects on liver functions.
PDF (English)

Parole chiave

Canine Hepatocyte
BCAA
decellularizatio
liver
scaffold

Abstract

Branched chain amino acids (BCAA), have been shown to affect human gene expression, protein
metabolism, apoptosis, and regeneration of hepatocytes. Furthermore, they have been demonstrated
to inhibit proliferation of liver cancer cells in vitro, and to be essential for lymphocyte proliferation.
In veterinary medicine, the use of BCAAs as integration of a normal dietary plan, is likely to be a valid
choice for the same benefit found in human clinical nutrition, although this aspect is still debated.
Indeed, long-term oral supplementation with BCAAs in the prevention of liver fibrosis and injury in the
dog's liver is still unclear. Aim of the present study will be to determine how BCAAs preserve liver
functions in vitro. To this purpose we have selected and set up three different in vitro models: hepatic
dog cells and canine hepatocellular carcinoma cells plated in 2D monolayer and hepatic dog cells
cultured onto 3D scaffolds, obtained from decellularized rabbit liver. All cells adhered and proliferated
once plated. Cells grown in monolayer quickly entered G0 end arrested growth, ELISA test confirmed
their ability to produce albumin. Cells grown on scaffold vigorously replicated and showed their
capability to recellularize ECM rabbit liver. These results, although preliminary, demonstrate that the
culture conditions used well preserved the original phenotype and function and further support the
possibility to use in vitro models to successfully study BCAA efficacy in dog.

https://doi.org/10.13130/2283-3927/7025
PDF (English)

Riferimenti bibliografici

Boomkens, S.Y., Spee, B., Ljzer, J., Kisjes, R., Egberink, H.F., Van Den Ingh, T.S., Rothuizen, J., Penning, L.C., 2004. The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns. Comp Hepatol. 3(1),9.

Duncan, A.W., Dorrell, C., Grompe, M., 2009. Stem Cells and Liver Regeneration. J Gastroenterol 137(2), 466–81.

Kruitwagen, H.S., Spee, B., Schotanus, B.A., 2014. Hepatic progenitor cells in canine and feline medicine: potential for regenerative strategies. BMC Vet Res. 10,137.

Rajkumar, R., Victor, R.P., Vinood., B.P., 2015. Branched Chain Amino Acids in Clinical Nutrition, New York, Humana Press.

Wakshlag, J.J., Kallfelz, F.A., Wakshlag, R.R., Davenport, G.M., 2006. The effects of branched-chain amino acids on canine neoplastic cell proliferation and death. J Nutr. 136(7), 2007S-2010S.

This work is licensed under a CC BY-SA 4.0 international