Antimicrobial susceptibility and glycopeptide-resistance of enterococci in vegetables

Abstract

Background: Vancomycin-resistant enterococci (VRE), often responsible for nosocomial infections, have
frequently been isolated from animal and vegetable foods. In our study we evaluated the antibiotic
susceptibility of enterococci isolated from eight types of vegetables randomly selected from grocery stores in
Naples.
Methods: From July to November 2008, we analyzed 150 samples: the bacteria were isolated with
standardized methods and antibiotic susceptibility was determined using the disc diffusion method. The
resistance to vancomycin versus other antibiotics was assessed by the Kappa test.
Results: 70% of the samples, mainly parsley (96.2%), showed enterococci. Of these, 59.1% belonged to the
species Enterococcus faecium. Strains resistant to vancomycin and teicoplanin were isolated respectively in
47.6% and 49.5% of the samples: the first one mainly in curly endive (72.7%) and the second one in parsley
(76.9%). Almost all the isolated strains showed resistance to methicillin (89%), kanamycin (82%) and
cephalothin (68%). The Kappa test showed statistically significant associations between resistance to
vancomycin and resistance to teicoplanin, erythromycin, methicillin, tetracycline and chloramphenicol.
Conclusions: Because of the possible involvement of food in the transmission of resistant micro-organisms to
human intestinal microbiota, our data may provide the basis for future studies.