Effect of inactivated cultures of Lactobacillus rhamnosus on subclinical mastitis quarter milk microbiota

  • Carlotta Catozzi Veterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy. http://orcid.org/0000-0002-1028-1489
  • Anna Cuscó Martí Vetgenomics. Ed Eureka. PRUAB. Campus UAB, Bellaterra, Barcelona, Spain.
  • Cristina Lecchi Veterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.
  • Valentina Zamarian Veterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.
  • Joaquim Viñes Pujol Vetgenomics. Ed Eureka. PRUAB. Campus UAB, Bellaterra, Barcelona, Spain.
  • Sara D'andreano Vetgenomics. Ed Eureka. PRUAB. Campus UAB, Bellaterra, Barcelona, Spain.
  • Alessandra Martucciello Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.
  • Giovanna Cappelli Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.
  • Carlo Grassi
  • Cinzia Marianelli Unit of Prophylaxis and Control of Bacterial Zoonoses. Department Of Veterinary Public Health and Food Safety. Istituto Superiore di Sanità. Viale Regina Elena 299, Rome, Italy.
  • Luisa D'Angelo Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.
  • Esterina De Carlo Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.
  • Domenico Vecchio Istituto Zooprofilattico Sperimentale del Mezzogiorno, National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions, Via delle Calabrie, Salerno, Italy.
  • Armand Sanchez Bonastre Molecular Genetics Veterinary Service (SVGM), Veterinary School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
  • Olga Francino Molecular Genetics Veterinary Service (SVGM), Veterinary School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain
  • Fabrizio Caciliani Veterinary Medicine Department, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.
Keywords: Water buffalo, Milk microbiota, Subclinical mastitis

Abstract

Water buffaloes mastitis represents a major issue in terms of animal health, cost of therapy, premature culling and decreased milk yeld. The emergence of antibiotic resistance has led to investigate strategies in order to avoid or minimize the antibiotic use, especially during subclinical mastitis disease (SM) (1). Lactobacillus rhamnosus is part of the normal gut microflora, having meanwhile an immunostimulatory activity (2). The aim of this study was to investigate the change of milk microbiota after the theraputic treatment with inactivated cultures of Lactobacillus rhamnosus of mammary gland quarters affected by subclinical mastitis. A number of 43 quarters were included in the study, and were treated with antibiotics, Lactobacillus rhamnosus  and PBS as negative control. Samples were collected at two time points, T0 and T5 (days) and V4 region of 16S rRNA gene was amplified by PCR and sequenced using Ion Torrent Personal Genome Machine. The microbiota structure of SM quarters showed no major changes after PBS treatment, while differed after antibiotic treatment where Staphylococcus decreased its relative abundance from 41% at T0 to 3% at T5. Lactobacillus rhamnosus induced a less dramatic change in milk microbiota, although the relative abundance of some genera were found to be modifidied, among which an increase of Pseudomonas from 1.5% at T0 up to 4% at T5. No differences were present between the microbiota structure of quarters treated with Lactobacillus rhamnosus and PBS. This study allowed to characterize the changes of microbiota in milk from animals with Lactobacillus rhamnosus and antibiotics. While changes in milk microbiota after antibiotic treatment were evident, changes after Lactobacillus rhamnosus were more limited. Further investigations are needed to evaluate alternative strategies to mastitis treatment. 

References

Moroni, P., Sgoifo Rossi, C.,, Pisoni, G., Bronzo V., Castiglioni, B., Boettcher PJ. 2006 Relationships between somatic cell count and intramammary infection in buffaloes. J. Dairy Sci. 89, 998-1003.

Fong, FL., Shah, NP., Kirjavainen, P., El-Nezami, H,. 2016 Mechanism of Action of Probiotic Bacteria on Intestinal and Systemic Immunities and Antigen-Presenting Cells. Int Rev Immunol. 35(3):179-88.

Published
2018-06-12
Section
Abstracts