Transcriptional regulation of lipid metabolism and inflammation in transition dairy goats by fish oil and stearate
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Parole chiave

Goat
Lipidic metabolism
Inflammation
Gene expression
Peripartum
Adipose tissue
Lipidic integration

Abstract

To better understand the interaction between saturated or unsaturated fatty acids and its effect on
expression of genes involved in subcutaneous adipose tissue metabolism, 23 second parity alpine dairy
goats were enrolled in the experiment and fed either a non fat-supplemented basal diet (C; n=8), the
basal diet supplemented with stearic acid (ST; n=7) or the basal diet supplemented with fish oil (FO;
n=8). 30g/head/d supplemental fatty acids during the dry period and 50g/head/d during lactation were
delivered starting one week before parturition up to 21 days in milk. Subcutaneous adipose tissue
samples were harvested at day -7, 7 and 21 relative to kidding and mRNA levels of genes involved in
inflammation were measured via qPCR. Data were analyzed using the MIXED procedure of SAS. No
significant effects for treatment were observed, however eight genes were significant for time. HP and
SAA3 expression peaked at day 7 postpartum, to then return at prepartum level around 21 d relative to
kidding, while IL8, IL10, and IL18 expression constantly increased along the transition period. Vice versa,
expression of IL1β, IL6R, and RXRA decreased in response to kidding, with a subsequent increase at day
21. The obtained results led us to hypothesize that goats face a postponed lipomobilization after
kidding, probably related to their reduced production. The next step will involve the analysis of miRNA
related to immune cell infiltration, adipocyte inflammation and lipolysis and positive regulation of
adipogenesis to better understand the complex network of lipid metabolism in periparturient goats.

https://doi.org/10.13130/2283-3927/7163
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Riferimenti bibliografici

Agazzi A., Cattaneo, D., Dell’Orto, V., Moroni, P., Bonizzi, L., Pasotto, D., and Savoini, G., 2004. Effect of administration of fish oil on aspects of cell-mediated immune response in periparturient dairy goats. Small Ruminant Research. 55, 77-83.

Calder, P.C., and Grimble, R.F., 2002. Polyunsaturated fatty acids, inflammation and immunity. Europ. J. Clincal Nutr. 56, S14-S19.

Invernizzi, G., Corbani, D., Caputo, J.M., Campagnoli, A., Pisani, L.F., Bronzo, V., Agazzi, A., Modina, S., and Savoini, G., 2012. Dietary fatty acids on subcutaneous adipose tissue modulation in transition dairy goats. Page 312 in Proc. XI International Conference on Goats, Gran Canaria, Spain.

Mallard, B.A., Dehhers, J.C., Ireland, M.J., Leslie, K.E., Sharif, S., Lacey Vankampen, C., Wagter, L., and Wilkie, B.N., 1998. Alteration in immune responsiveness during the peripartum period and its ramification on dairy cow and calf health. Journal of Dairy Science. 81, 585–594.

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