Meniscus matrix morphological composition: age-dependent evaluation in a swine model

  • Umberto Polito Università degli Studi di Milano - Faculty of Veterinary Medicine - Department of Health, Animal Science and Food Safety - Via Trentacoste, 2 - 20134 - Milano
  • Elena Dell'Era freelance DVM
  • Irene Tessaro IRCCS istituto ortopedico Galeazzi, via Riccardo Galeazzi, 4 - 20161 - Milano
  • Giuseppe Maria Peretti IRCCS istituto ortopedico Galeazzi, via Riccardo Galeazzi, 4 - 20161- Milano
  • Alessia Di Giancamillo Università degli Studi di Milano - Faculty of Veterinary Medicine - Department of Health, Animal Science and Food Safety - Via Trentacoste, 2 - 20134 - Milano
Keywords: Meniscus, pig, matrix

Abstract

Menisci are fibro-cartilaginous structures interposed between femoral condyle and tibial plateau, which have multiple functions in the stifle joint: act as shock absorbers, bear loaders and allow joint stability, congruity and lubrication (Sweigart et al., 2004; Proffen et al., 2012). It is well known that meniscal injuries lead to osteoarthritis and for these reasons, menisci are considered important target of investigation. Their important role in the knee wellness is only equalled by their deficiency in proper self-repairing.

Nowadays, the gold standard technique is not just to remove the damaged meniscus, but to rebuild it or to replace it. For these reasons, studies are necessary to increase the knowledge about these small but essential structures (Streuli, 1999; Deponti et al., 2013). Composition and morphology are basic fundamental information for the development of engineered meniscal substitutes (Di Giancamillo et al., 2014). The analysis of the morphological, structural and biochemical changes, which occur during growth of the normal menisci, represent the goal of the present study. For this purpose, menisci from adult (7-month old), young (1-month old), and neonates (stillbirths) pigs were collected. Cellularity and glycosamiglycans (GAGs) deposition were evaluated by ELISA, while Collagen-1 and Collagen-2 were investigated by immunohistochemistry and Western blot analyses. Cellularity (P<0.01, all comparisons) and Collagen-1 (P<0.05, neonatal-young vs adult) decreased from neonatal to adult stage while GAGs (P<0.01 neonatal vs young-adult) and Collagen-2 (P<0.01 neonatal-young vs adult) showed the opposite trend. Immunohistochemistry revealed similar changes occurring during animal growth thus revealing that cellular phenotype, cellularity and protein expression, as well as fibers aggregation in the matrix, are dissimilar in the three ages analysed categories. These changes reflect the progressive menisci maturation and hyper-specialisation. We observed the correlation between biochemical and phenotype properties of swine menisci follow age-dependent changes during growth: starting with an immature cellular and fiber pattern to the mature organised and differentiated adult menisci.

Acknowledgments: This work was funded by the “Finanziamento Piano Sviluppo Ateneo - Linea 2A”

References

Deponti D, Di Giancamillo A, Scotti C, Peretti GM and Martin I: Animal models for meniscus repair and regeneration; J Tissue Eng Regen Med, 2013;

Di Giancamillo A, Deponti D, Addis A, Domeneghini C, Peretti GM: Meniscus maturation in the swine model: changes occurring along with anterior to posterior and medial to lateral aspect during growth; J. Cell. Mol. Med. 2014; Vol 18, n°10, pp. 1964-1974;

Proffen BL, McElfresh M, Fleming BC, Murray M: A comparative anatomical study of the human knee and six animal species; The Knee 2012; 19; 493–499;

Streuli C: Extracellular matrix remodelling and cellular differentiation; Current Opinion in Cell Biology, 1999; 11:634–640;

Sweigart MA, Zhu CF, Burt DM, Deholl PD, Agrawal CM, Clanton TO, Athanasiou KA; Intraspecies and Interspecies Comparison of the Compressive Properties of the Medial Meniscus; Annals of Biomedical Engineering, 2004; Vol 32, n 11, pp. 1569–1579;

Published
2017-06-01
Section
Articles