Molecular typing of Staphylococcus pseudintermedius canine strains by three commonly used techniques


Staphylococcus pseudintermedius
molecular typing
Ribosomal Spacers Amplification
Restriction Fragment Length Polymorphism
Random Amplification of Polymorfic DNA


Staphylococcus pseudintermedius is a newly described species of Staphylococcus regarded as the main causative agent of canine pyoderma [1]. S. pseudintermedius infection was recently described in humans. An important feature of this pathogen is the high genetic identity with two other species of staphylococci, namely S. intermedius and S. delphini, which are included all together in the Staphylococcus Intermedius Group (SIG) [2]. This scenario seriously hampers phenotypic differentiation of these three pathogens. Despite this, only in 2008 was described the first molecular protocol for diagnostic identification of   S. pseudintermedius [3]. The aim of this work was to investigate the presence of different biotypes of S. pseudintermedius obtained from clinically relevant cases of pyoderma in dogs using three molecular methods commonly used to type bacteria: the Ribosomal Spacers Amplification (RSA), the Random Amplification of Polymorphic DNA (RAPD) and the Restriction Fragment Length Polymorphism (RFLP). A total of 46 different strains were included in this work. The application of the RSA technique, which was applied here for the first time, identified the presence of S. pseudintermedius, although it did not allow any differentiation between biotypes. The RAPD assay showed a single cluster that assembles all the interested strains that are grouped in three different sub-clusters (Fig. 1). The RFLP technique showed the most discriminative power, providing the opportunity to clearly identify this bacterium. In conclusion, the use of these three different techniques allows to clearly identify S. pseudintermedius and to observe the presence of different biotypes. In future it could be interesting to couple these results with the determination of the antibiotic resistance in order to verify if certain Multi Drug Resistant strains have particular RSA and RAPD profiles.


Bannoehr J, Franco A, Iurescia M, Battisti A, Fitzgerald JR., 2009. Molecular Diagnostic Identification of Staphylococcus pseudintermedius. Journal of Clinical Microbiology.47(2),469-471.

Devriese, L. A., Vancanneyt, M., Baele, M., Vaneechoutte, M., De Graef, E., Snauwaert, C., Haesebrouck, F., 2005. Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals. International Journal of Systematic and Evolutionary Microbiology. 55(Pt 4), 1569-1573.

Fitzgerald, J. R. 2009. The staphylococcus intermedius group of bacterial pathogens: Species re-classification, pathogenesis and the emergence of meticillin resistance. Veterinary Dermatology. 20, 490-495.

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