Effect of phytase supplementation on apparent phosphorus digestibility and phosphorus output in broiler chicks fed low-phosphorus diets

  • Xian-Ren Jiang Feed Research Institute, Chinese Academy of Agricultural Sciences
  • Fa-Hong Luo Feed Research Institute, Chinese Academy of Agricultural Sciences
  • Shu-Geng Wu Feed Research Institute, Chinese Academy of Agricultural Sciences
  • Hai-Jun Zhang Feed Research Institute, Chinese Academy of Agricultural Sciences
  • Valentino Bontempo Department of Health, Animal Science and Food Safety, Università degli Studi di Milano
  • Ming-Ren Qu Animal Science and Technology College, Jiangxi Agricultural University
  • Hong-Yuan Yue Feed Research Institute, Chinese Academy of Agricultural Sciences
  • Guang-Hai Qi Feed Research Institute, Chinese Academy of Agricultural Sciences
Keywords: Apparent phosphorus digestibility, Chicks, Low-phosphorus, Phosphorus output, Phytase

Abstract

This study was conducted to evaluate the effect of supplemental phytase in broiler chicks fed different low levels of total phosphorus (P) on the apparent phosphorus digestibility (APD) and phosphorus output (PO) in the faeces and ileal digesta. After fed a standard broiler starter diet from day 0 to 14 post-hatch, a total of 144 male broiler chicks were allocated to 6 groups for a 7-d experiment with a 2 × 3 factorial design comparing phytase (supplemented without (CTR) or with 400 FTU/kg phytase (PHY)) and total P levels (2.0, 2.5 and 3.0 g/kg). The faecal samples were collected from day 17 to 21 post-hatch. At 22 days of age, all the chicks were slaughtered and collected the ileal digesta. Phytase supplementation significantly (P < 0.01) increased APD and decreased PO in the faeces and ileal digesta in comparison with the CTR group. In addition, PO in the faeces expressed as g/kg DM diets and faeces (Diet × P level, P = 0.047 and < 0.01, respectively) as well as PO in the ileal digesta expressed as g/kg DM digesta (Diet × P level, P = 0.04) were affected by diet and P level, which were due to the significant reduction (P < 0.01) by PHY supplementation to the diets with 3.0 g/kg total P. The results evidenced that supplemental phytase improved the APD and PO when chicks was fed 3.0 g/kg total P diet, while lower total P levels may limit exogenous phytase efficacy.

References

Abudabos M.A., 2012. Phytate phosphorus utilization and intestinal phytase activity in laying hens. Ital. J. Anim. Sci., 11:e8.

AOAC, 2005. Official Methods of Analysis, 18th ed., Association Official Analytical Chemists, Arlington, VA, USA.

Attia, Y.A., El-Tahawy, W.S., Abd El-Hamid, A.E., Hassan, S.S., Nizza, A., El-Kelaway, M.I., 2012. Effect of phytase with or without multienzyme supplementation on performance and nutrient digestibility of young broiler chicks fed mash or crumble diets. Ital. J. Anim. Sci., 11:e56.

Biehl, R.R., Baker, D.H., 1996. Efficacy of supplemental 1 alpha-hydroxycholecalciferol and microbial phytase for young pigs fed phosphorus- or amino acid-deficient corn-soybean meal diets. J. Anim. Sci. 74:2960-2966.

Dilger, R.N., Adeola, O., 2006. Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing chicks fed conventional and low-phytate soybean meals. Poult. Sci. 85:661-668.

Jiang, X.R., Luo, F.H., Qu, M.R., Bontempo, V., Wu, S.G., Zhang, H.J., Yue, H.Y., Qi, G.H., 2013. Effect of non-phytate phosphorus levels and phytase sources on the growth performance, serum biochemical and tibial parameters of broiler chickens. Ital. J. Anim. Sci. 12:e60.

National Research Council, 1994. Nutrient Requirements of Poultry, 9th rev. ed. Natl. Acad. Press, Washington, DC, USA.

Nelson, T.S., Sheih, T.R., Wodzinski, R.J., Ware, J.H., 1968. The availability of phytate phosphorus in soybean meal before and after treatment with a mold phytase. Poult. Sci. 47:1842-1848.

Paik, I.K., 2003. Application of phytase, microbial or plant origin, to reduce phosphorus excretion in poultry production. Asian-Aust. J. Anim. Sci. 16:124-135.

Panda, A.K., Rama, Rao S.V., Raju, M.V.L.N., Gajula, S.S., Bhanja, S.K., 2007. Performance of broiler chickens fed low non phytate phosphorus diets supplemented with microbial phytase. J. Poult. Sci. 44:258-264.

Rutherfurd, S.M., Chung, T.K., Morel, P.C., Moughan, P.J., 2004. Effect of microbial phytase on ileal digestibility of phytate phosphorus, total phosphorus, and amino acids in a low-phosphorus diet for broilers. Poult. Sci. 83:61-68.

Rutherfurd, S.M., Chung, T.K., Thomas, D.V., Zou, M.L., Moughan, P.J., 2012. Effect of a novel phytase on growth performance, apparent metabolizable energy, and the availability of minerals and amino acids in a low-phosphorus corn-soybean meal diet for broilers. Poult. Sci., 91:1118-1127.

SAS Institute, 2008, SAS/STAT User’s Guide, Version 9.2. SAS Institute, Inc., Cary, NC, USA.

Scott, M.L., Nesheim, M.C., Young, R.J., 1982. Nutrition of the chicken. 3rd ed. Scott M.L. & Associates Publ., Ithaca, NY, USA.

Sebastian, S., Touchburn, S.P., Chavez, E.R., Lague, P.C., 1996. The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper and zinc in broiler chickens fed corn-soybean diets. Poult. Sci. 75:729–736.

Shieh, T.R., Ware, J.H., 1968. Survey of microorganisms for the production of extracellular phytase. J. Appl. Microbiol. 16:1348-1351.

Viveros, A., Brenes, A., Arija, I., Centeno, C., 2002. Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poult. Sci. 81:1172–1183.

Waldroup, P.W., Kersey, J.H., Saleh, E.A., Fritts, C.A., Yan, F., Stilborn, H.L., Crum, Jr R.C., Raboy, V., 2000. Nonphytate phosphorus requirement and phosphorus excretion of broiler chicks fed diets composed of normal or high available phosphate corn with and without microbial phytase. Poult. Sci. 79:1451-1459.

Published
2015-04-26
Section
Articles