Effects of essential oil from Cymbopogon citratus leaves and its susceptibility on the quality of fresh orange juice during storage
The present study aims to evaluate the effect of essential oil (EO) from Cymbopogon citratus leaves against the spoilage flora of fresh orange juice. Thus, the EO was extracted by hydrodistillation from fresh leaves of Cymbopogon citratus collected in southern Benin and its chemical composition was determined by gas chromatography, coupled to mass spectrometry (GC/MS). Orange samples were collected from large production areas of South and Central Benin and juices were extracted by mechanical pressing. After identification of spoilage flora of fresh orange juice, antimicrobial tests were carried out with the EO of Cymbopogon citratus to evaluate its antimicrobial activity on spoilage flora of fresh orange juice. Results indicate that the spoilage flora of fresh orange juice is mainly composed of fungi belonging to the genera of Cladosporium, Penicillium and Fusarium. Bacteria such as Enterobacter cloacae and Enterobacter aerogenes were also identified in some samples. The major compounds identified in the EO by GC/MS are Neral (33.0%) and geranial (41.3%) with a predominance of oxygenated monoterpenes (85.5%). Antimicrobial tests have revealed a high antibacterial activity of the EO, with minimum bactericidal concentrations (MBC) between 0.1 and 0.15 μL.mL-1. Antifungal tests revealed that fungi are also susceptible to this EO with minimum fungicidal concentration (MFC) between 0.15 and 0.25 μL.mL-1. Results obtained during the evaluation of the physicochemical characteristics of the orange juice stored by adding EO, indicated a significant decrease in the pH and vitamin C content. However, with EO concentration of 0.250 μL.mL-1, the pH of stored juice was 6.4 ± 0.1 after 15 days of preservation, with a best vitamin C content of 28.06 ± 0.03 mg / 100mL. The EO of Cymbopogon citratus, with high antimicrobial activity, could be used as an alternative in the preservation of fruit juices, replacing antimicrobials from chemical synthesis.
Adams R.P., 2007. Identification of essential oil components by gas chromatography / mass spectrometry. Allured, Carol Stream.
Adjou E. S., Amamion H., Tchobo F. P., Aissi V. M., Soumanou M.M., 2013. Extraction assistée par enzyme du jus de la pulpe fraîche du rônier (Borassus aethiopum Mart.) acclimaté au Benin : caractérisation physico-chimique et microbiologique. Int. J. Biol. Chem. Sci. 7(3), 1135-1146.
Adjou E.S., Yehouenou B., Sossou C.M., Soumanou M.M., de Souza C.A., 2012. Occurrence of mycotoxins and associated mycoflora in peanut cakes products (kluiklui) marketed in Benin. Afr J Biotechnol 11(78), 14354–14360.
Bajpai K.V., Dung N.T., Kwon J.O., Kang S.C., 2008. Analysis and the potential application of essential oil and leaf extracts of Silene Americana L. to control food spoilage and food-borne pathogens. J. Food Technol., 227: 1613-1620.
Bankole S., Mabekoje O.O., 2004. Mycoflora and occurrence of aflatoxin B1 in dried yam chips from markets in Ogun and Oyo States, Nigeria. Mycopathologia, 157, 111-115.
Barminas J.T., Charles M., Emmanuel D., 1998. Mineral Composition of Non- conventional Leafy Vegetables. Plant Foods for Human Nutrition 53 (1), 29-36.
Bazemore R., Goodner K., Rouseff R., 1999. Volatiles from Unpasteurized and Excessively Heated Orange Juice Analyzed with Solid Phase Microextraction and GC-Olfactometry. J. Food. Sci. 64 (5), 800-803.
Dahouenon-Ahoussi E., Degnon R. G., Adjou E. S., Sohounhloue D.C.K., 2012. Stabilisation de la bière produite à partir de matières amylacées locales (Sorghum bicolor et Musa acuminata) par adjonction de l’huile essentielle de Cymbopogon citratus. Journal of Applied Biosciences 51, 3596 – 3607.
de Billerbeck V.G., Roques C.G., Bessière J.M., Fonvieille J.L, Dargent R., 2001. Effect of Cymbopogon nardus (L) W. Watson. essential oil on the growth and morphogenesis of Aspergillus niger. Can J Microbiol 47, 9–17.
Dorman H.J.D., Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils, J. Appl.Microbiol., 88, 308–316. 2000.
Esteve J.M., Frigola A., 2008.The effects of thermal and Non-thermal Processing on Vitamin C, Carotenoids, Phenolic Compounds and Total Antioxydant Capacity in Orange juice. Tree and forestry Science and Biotechnology, 2(1), 128-134.
FAO/WHO, 1998. Forty-ninth meeting of the Joint Expert Committee on Food Additives. Food Agric. Organ. United Nation. Rome, p. 140.
Hammer K.A., Carson C.F., Ridley C.V., 1999. Antimicrobial activity of essential oils and other plants extract. Journal of Applied Microbiology, 86, 985-990.
Hidalgo E., Moor, D., Patourel G., 1998.The effect of different formulations of Beauveria bassiana on Sitophilus zeamais. Journal of Stored Products Research, 34, 171–179.
Leclerc H., Schwarrtzbrod L., Dei-Cas E., 2002. Microbial agents associated with waterborne diseases. Crit. Rev. Microbiol., 28,371-409.
Matasyoh J.C., Wagara I.N., Nakavuma J.L., Kiburai A.M., 2011. Chemical composition of Cymbopogon citratus essential oil and its effect on mycotoxigenic Aspergillus species. African Journal of Food Science, 5(3), 138-142
Melo S.F., Soares S.F., Costa D.R., Silva D.C., Oliveira D.M., Bezerra R.J., 2001. Effect of the Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts against the stannous chloride oxidative damage in Escherichia coli. Mutat Res., 496, 33–41.
Misrha A.K., Dubey N.K., 1994. Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Appl Environ Microbiol., l60, 1101–1106.
Moosavy M.H., Basti A.A., Ali M., 2008. Effect of Zataria multiflora Boiss. Essential oil and nisin on Salmonella typhimurium and Staphylococcus aureus in a food model system and on the bacterial. International Journal of Food Microbiology, 43, 69–76.
Oussou K.R., Kanko C., Guessend N., Yolou, S., Koukoua G., Dosso M., N’guessan Y.T., Figueredo G., Chalchat J-C., 2004. Activités antibactériennes des huiles essentielles de trois plantes de Côte d’Ivoire. C.R. Chim., 7, 1081-1086.
Prakash B., Shukla R., Singh P., Mishra PK., Dubey N.K., Kharwar R.N., 2010. Efficacy of chemically characterized Ocimum gratissimum L. essential oil as an antioxidant and a safe plant based antimicrobial against fungal and aflatoxin B1 contamination of spices, Food Res. Int., 10, 128 -132.
Singh K., Frisvad J.C., Thrane U., Mathu S.B., 1991. An illustrated manual on identification of some seed borne Aspergilli, Fusaria, Penicillia and their mycotoxins. Heller up, Denmark: Danish Government, Instituteof seed pathology for developing countries. p 133.
Somogyi L.P., 1996. Direct food additives in food processing. In L. P. Somogyi, D. M. Barrett, & Y. H. Hui (Eds.), Processing Fruits: Science and Technology, Biology, Principles, and Application. Western Hemisphere 7 Technomic Publishing, 1, 342-345.
Soumanou M.M. and Adjou E.S., 2016. Sweet Fennel (Ocimum gratissimum) Oils. In:Preedy, V.R. (Ed.), Essential Oils in Food Preservation, Flavor and Safety. Academic Press, 765–773.
Suhr K.I., Nielsen P.V., 2003. Antifungal activity of essential oils evaluated by two different application techniques against rye bread spoilage fungi, J. Appl. Microbiol., 94(4), 665–674.
Tomohiro S., 1990. Laboratory Manual for Food analysis”, Technical Cooperation Project of Jomo Kenyatta 76p.
Tran M. T. T., Farid M., 2004. Ultraviolet treatment of orange juice. Innovative Food Science and Emerging Technologies 5, 495– 502.
Voda K., Boh B., Vrta-cnik M., Pohleven F., 2003. Effect of the antifungal activity of oxygenated aromatic essential oil compounds on the white-rot Trametes versicolor and the brown-rot Coniophora puteana, Int. Biodeterior. Biodegrad., 51, 51–59.
Yèhouenou B., Noudogbèssi J.P., Sessou P., Avlessi F., Sohounhloué D., 2010. Etude chimique et activités antimicrobiennes d’extraits volatils des feuilles et fruits de Xylopia aethiopica (DUNAL) A. Rich. contre les pathogènes des denrées alimentaires. J. Soc Ouest – Afr. Chim., 029, 19-27.
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