Evaluation of the fungal microflora infesting pigeon pea (Cajanus cajan L. Millspaugh) in southern Benin and associated mycological hazards


pigeon pea
mycological hazards


Pigeon pea is a perennial legume with a good nutritional value. Unfortunately, it is also a substrate for fungi contamination. Then, a qualitative semi-structured survey was carried out in the main production areas of pigeon pea in southern Benin. This survey was coupled with samples collection. A total of 60 samples of pigeon pea were collected and analyzed for associated fungal microflora by using a taxonomic schemes primarily based on morphological characters of mycelium and conidia. Obtained results indicated a low technological valorization of pigeon pea seeds in southern Benin and their used only in direct consumption after cooking. Microbiological analyses revealed the high contamination of pigeon pea seeds by fungi, with the most occurrence of Aspergillus (71.42%), followed by Fusarium (26.19%). Fungal species such as Aspergillus ochraceus, A. parasiticus, A. flavus and Fusarium oxysporum were also detected in analyzed samples. Taking into account the toxicity of the secondary metabolites produced by these fungi, mycological hazards are discussed and important methods for the control of mycotoxin-contamination are further provided. More attention should be paid to the mycological quality of this legume, in order to protect the consumers’ health.



Ade-Omowaye, B.I.O., Tucker, G.A., Smetanska, I, 2015. Nutritional potential of nine underexploited legumes in Southwest Nigeria. International Food Research Journal 22 (2), 798-806.

Adjou, E.S., Yehouenou, B., Sossou, C.M., Soumanou, M.M., de Souza, C.A., 2012. Occurrence of mycotoxins and associated mycoflora in peanut cakes products (kluiklui) marketed in Benin. Afr J Biotechnol 11(78), 14354–14360.

Adjou, E.S., Kouton S., Dahouenon-Ahoussi E., Soumanou M. M., Sohounhloue D. C. K., 2013. Effect of essential oil from fresh leaves of Ocimum gratissimum L. on mycoflora during storage of peanuts in Benin. Mycotoxin Res., 29, 29–38.

Almeida-Costa, G.E., Queiroz-Monici, K.S., Machado- Reis, S.M.P. and Oliveira, A.C., 2006. Chemical composition, dietary fibre and resistant starch contents of raw and cooked pea, common bean, chickpea and lentil legumes. Food Chemistry 94, 327–330.

Amarteifio J.O., Munthali D.C., Karikari S.K., Morake T.K. The composition of pigeonpea (Cajanus cajan (L.) Millsp.) grown in Botswana. Plant Foods for Human Nutrition, 57 (2002) 173-177.

Boehringer, A., Cadwel, R., 1989. Cajanus cajan (L.) millsp. As potentiel agroforestry component in the eastern province of Zambia. Agroforest. syst. 9, 127-140.

Cahagnier B., Dragacc S., Frayssinet C., Frémy J.M., Hennebert G.L., Lesage-meessen L., Multon J.L., Richard-Molard D., Roquebert M.F., 1998. Moisissures des aliments peu hydrates. Lavoisier Tec&Doc, France, 63p.

Castegnaro, M., 1999. Risques cancérogènes - Les aflatoxine. Dans “Les mycotoxines dans l’alimentation: évaluation et gestion du risque” de conseil supérieur d’hygiène publique de France. Technique et Documentation, Paris, 35p.

CEC,1998. Commission Regulation (EC) No. 1525/98. Official Journal of European Communities L20/143

El-Adlouni, C., Tozlovanu, M., Natman, F., Faid, M., Pfohl-Leszkowicz, A., 2006. Preliminary data on the presence of mycotoxins (ochratoxin A, citrinin and aflatoxin B1) in black table olives ‘‘greek style” of Moroccan origin. Mol. Nutr. Food Res. 50, 507–512.

Fernández-Cruz, M L., Mansilla, M.L., Tadeo J.L., 2010. Mycotoxins in fruits and their processed products: Analysis, occurrence and health implications. Journal of Advanced Research 1, 113–122.

Filtenborg, O., Frisvad, J.C., Thrane, U., 1995. Moulds in food spoilage. Int J Food Microbiol 33, 85–102.

Fossou, R.K., Kouassi, N.K., Kouadjo, G.C.Z., Zako, S. M. I. B., Zeze, A., 2012. Diversité de rhizobia dans un champ cultivé de pois d’Angole (Cajanus cajan l.,) à yamoussoukro (Côte d’Ivoire). Agronomie Africaine 24 (1), 29 - 38.

Hubert J., Steyskal V., Munzbergova Z., kubatova A., Vanovall., Zd’arkova E., 2007. Mites and fungi in heavily infested stores in the Czech Republic, J. Entomol., 97(6): 2144-2153.

Kamanga, B.C.G., Shamudzarira, Z., 2001. On-farm legume experimentation to improve soil fertility in Zimuto communal area, Zimbabwe: Farmer perceptions and feedback. Seventh Eastern and Southern Africa regional Maize Conference, 11–15p.

Molinie, A., Faucet, V., Castegnaro, M., Pfohl-Leszkowicz, A., 2005. Analysis of some breakfast cereals on the French market for their contents of ochratoxin A, citrinin and fumonisin B1: development of a method for simultaneous extraction of ochratoxin A and citrinin. Food Chem. 92, 391-400.

Moss, M.O., 1989. Mycotoxins of Aspergillus and other filamentous fungi. J. Appl. Bacteriol. 67(Symposium Suppl), 695-815.

Naylor, R.L., Falcol, W.P., Goodman, R.M, Jahn, M.M, Sengooba, T., Tefera, H., Nelson, R.J., 2004. Biotechnology in the developing world: A case for increased investments in orphan crops. Food Policy 29, 15–44.

Nestel, P., Cehun, M., and Chronopoulos, A., 2004. Effects of long-term consumption and single meals of chickpeas on plasma glucose, insulin, and triacylglycerol concentrations. The American Journal of Clinical Nutrition 79, 390-395.

Nguyen, M.T., 2007. Identification des espèces de moisissures potentiellement productrices de mycotoxines dans le riz commercialisé dans cinq provinces de la région centrale du Vietman : Etude des conditions pouvant induire la production de mycotoxines. Thèse de doctorat, Institut National Polytechnique de Toulouse (INPT), Toulouse, 147p.

Pazhamala L., Saxena, R.K., Singh, V.K., Sameerkumar, C.V., Kumar, V., Sinha, P., Patel, K., Obala, J., Kaoneka, S.R., Tongoona, P., Shimelis, H. A., Gangarao, N.V.P.R., Odeny, D., Rathore, A., Dharmaraj, P.S., Yamini, K.N., Varshney, R.K., Genomics-assisted breeding for boosting crop improvement in pigeon pea (Cajanus cajan). Front Plant Sci. 6, 50p

Pfohl-Leszkowicz, A., 2002. Définition et origines des mycotoxines. Les mycotoxines dans l’alimentation: évaluation et gestion du risque, Ed. Tec et Doc, 3-14p.

Pitt, J.I., Hocking, A.D., Bhudhasamai, K., Miscamble, B.F., Wheeler, K.A., Tanboon, E.K.P., 1994. The normal mycoflora of commodities from Thailand: beans, rice, small grains and other commodities. Int J Food Microbiol 23, 35–53.

Singh, K., Frisvad, J.C., Thrane, U., Mathu, S.B., 1991. An illustrated manual on identification of some seed borne Aspergilli, Fusaria, Penicillia and their mycotoxins. Danish Government, Institute of seed pathology for developing countries, Hellerup, Denmark

Sultan, Y., Magan, N., 2010. Mycotoxigenic fungi in peanuts from different geographic regions of Egypt. Mycotox Res 26, 133–140.

Swart, W.J., Mathews, C., Saxena, K.B., 2000. First report of leaf rust caused by Uredo cajan ion pigeon pea in South Africa. Plant Disease 84, 1344p.

Tabuc, C., 2007. Flore fongique de différents substrats et conditions optimales de production des mycotoxines. Thèse de doctorat, Institut National Polytechnique de Toulouse et Université de Bucarest, Toulouse, 156p.

Velay, F., Baudoin, J-P., Mergeai, G., 2001. Caractérisation du savoir paysan sur les insectes nuisibles du pois d’Angole (Cajanus cajan (L.) Millsp.) dans le Nord de l’Ouganda. Biotechnol. Agron. Soc. Environ. 5 (2), 105–114.

Versteeg, M.N., Koudokpon, V., 1993. Participative farmer testing of four low external input technologies to address soil fertility decline in Mono province (Benin). Agricultural Systems, 42, 265 –276.

Wu, N., Fu, K., Fu, Y.J., Zu, Y.G., Chang, F.R., Chen, Y.H., Liu, X.L., Kong, Y., Liu, W., Gu, CB., 2009. Antioxidant activities of extracts and main components of pigeon pea (Cajanus cajan L. Millsp). leaves. Molecule 14 (3), 1032-1043.

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