Compared the effect of indirect ELISA and serum plate agglutination (SPA) test for the detection of Mycoplasma gallisepticum in chicken


Mycoplasma gallisepticum (MG) is a highly economical and persistent threat of poultry industry in Bangladesh. Indirect ELISA (iELISA) and Serum plate agglutination test (SPA) is available serological test for diagnosis of MG antibodies. The aim of this research was conducted on the basis of comparison on diagnosis results between iELISA and SPA test for MG antibody in same sample in layer chicken. Total 563 serum samples were collected and tested for MG antibody by both iELISA and SPA test. Out of 563 samples 363 (64.48%) samples were positive by iELISA and 316 (56.13%) samples were positive in SPA test. The higher incidence of MG antibody was found in chicken at 50-56 weeks and flock size was 3000-4200 as 69.63% by iELISA and 61.21% by SPA and in Sonali breeds 69.08% by iELISA and 60.64% by SPA. The results showed the comparatively higher number of positive results in iELISA test than SPA test. So the findings of the study demonstrated that a significant (p<0.05) difference between iELISA and SPA test present. The study may helpful for screening the flock for MG and small-holding farmers may use SPA test rather than iELISA test due to rapid, easy and cost effective.


Ali, M.Z., Rahman, M.M., Sultana, S. 2015. Seroprevalence of Mycoplasma gallisepticum antibody by ELISA and serum plate agglutination test of laying chicken. Veterinary World. 8(1), 9-14.

Arefin, M., Begum, J.A., Parvin, R., Rahman, M.M., Khan, M.A.H.N.A., Chowdhury, E.H. 2011. Development of slide agglutination test for the rapid diagnosis of Mycoplasma infection in the chicken. Bangladesh Veterinarian. 28(2), 80-84.

Avakian A.P., Kleven, S.H., Glisson, J.R. 1988. Evaluation of the specificity and sensitivity of two commercial ELISA kits, the SPAT, and the HI test for antibodies formed in response to Mycoplasma gallisepticum. Avian Diseases. 32, 262-272.

Butcher, G.D. 2002. Factors to consider in serologic testing for Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). University of Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences. 126, 1-2. Available at:

Feberwee, A., Mekkes, D.R., De Wit, J.J., Hartman, E.G., Pijpers, A. 2005. Comparison of culture, PCR and different serologic tests for detection of Mycoplasma gallisepticum and Mycoplasma synoviae infections. Avian Diseases. 49(2), 260-268.

Feizi, A., Khakpour, M., Nikpiran, H., Kaboli, K., Bijanzad, P. Moggadam, A.R.J., Hosseini, H. 2013. Comparative Evaluation of Serological test in Diagnosis of Mycoplasma gallisepticum Infection in Iranian North-west rural Poultry. Advances in Bioresearch. 4(3), 50-53.

Heleili, N., Ayachi, A., Mamache, B., Chelihi, A.J. 2012. Seroprevalence of Mycoplasma synoviae and Mycoplasma gallisepticum at Batna Commercial poultry farms in Algeria. Veterinary World. 5(12), 709-712.

Hossain, K.M.M., Ali, M.Y., Haque, M.I. 2007. Seroprevalence of Mycoplasma gallisepticum infection in chicken in the greater Rajshahi district of Bangladesh. Bangladesh Journal of Veterinary Medicine. 5 (1 & 2), 09-14.

Jalilnia, M., Movassagh, M.H. 2011. A study on causes of poultry carcasses condemnation in East Azerbaijan province (North West of Iran) poultry slaughterhouse. Annals of Biological Research. 2(4): 343-347.

Kempf, I and Gesbert, F. 1998. Comparison of serological tests for detection of Mycoplasma gallisepticum antibodies in eggs and chicks hatched from experimentally infected hens. Veterinary Microbiology. 60(2), 207-213.

Kempf, I., Gesbert, F., Guittet, M., Bennejean, G., Stipkovits, L. 1994. Evaluation of two commercial enzyme-linked immunosorbent assay kits for the detection of Mycoplasma gallisepticum antibodies. Avian Pathology. 23, 329-338.

Ley, D.H. 2008. Mycoplasma gallisepticum Infection, Eds., Saif, Y.M. Disease of Poultry, Wiley-Blackwell Publishing, Iowa, IA, pp: 807-834.

Luciano, R.L., Cardoso, A.L.S.P., Stoppa, G.F.Z., Kanashiro, A.M.I., De Castro, A.G. M., Tessari, E.N.C. 2011. Comparative study of serological tests for Mycoplasma synoviae diagnosis in commercial poultry breeders. Veterinary Medicine International. 2011, 1-5.

Nascimento, E.R. 2000. Micoplasmoses in Doenc¸a das Aves, Macari, M. and Berchieri, A., Jr., Eds., Campinas, Sao Paulo, Brazil, pp. 217-224

OIE. 2015. Avian mycoplasmosis (Mycoplasma gallisepticum, M. synoviae) in Manual of diagnostic tests and vaccines for terrestrial animals. World Organisation for Animal Health (OIE), Paris, France.

Pourbakhsh, S.A., Shokri, G.R., Banan, M., ELHamnia, F., Ashtari, A. 2010. Detection of Mycoplasma synoviae infection in broiler breeder farms of Tehran province using PCR and culture methods. Archives of Razi Institute. 65, 75-81.

SAS (1996). SAS/STAT User’s Guide: Statistics. Version 6, 4th Edn., SAS Institute Inc., Cary, NC.

Sikder, A.J., Islam, M.A., Rahman, M.M., Rahman, M.B. 2005. Seroprevalence of Salmonella and Mycoplasma gallisepticum Infection in the Six Model Breeder Poultry Farms at Patuakhali District in Bangladesh. International Journal of Poultry Science. 4(11), 905-910.

Stipkovits, L., Czifra, G., Sundquist, B. 1993. Indirect ELISA for the detection of a specific antibody response against Mycoplasma gallisepticum. Avian Pathology. 22(3), 481-494.

Yoder, H.W. 1991. Mycoplasma gallisepticum infection. In: Calnek, B.W., Beard, C.W., Barnes, H.J., Reid, W.M., Yoder, H.W., Jr. Eds., Diseases of Poultry. 9th 3dn. Iowa State Univ. Press, Ames, IA, pp. 198-212.

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