The aim of this research is to examine DJ set performance practices within queer subjectivities and communities. We analyze development of queer music cultures and scenes as a synergy between musical and sexual dimensions (Taylor 2008, 2012), grounded in the inclusion and recognition of musical preferences and identities. Special attention is given to electronic music as a site for transfeminist and queer experimentation in music, technology, arts (Attimonelli 2018; Attimonelli, Tomeo 2022), as well as the social function of the DJ set, investigating sound both as an artifact and as a space. Methodology is based on ethnographic moments and interviews with key figures from a local scene, each characterized by distinct trajectories, experiences, and modes of expression. By applying a gender lens, this analysis uncovers forms of activism that operate outside of sexist logics, alongside practices of queering and renegotiating spaces in the pursuit of creating safer spaces.